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#4PEAKS SOFTWARE DOWNLOAD WINDOWS TV#
It does not store any personal data.Ok, by now, you should have downloaded PC1.11 and have the software to analyze it (4peaks for macs, Finch TV for windows) You should also know about how the DNA is sequenced and what to look for when first analyzing the gene (the machine records the nucleotide in a sequence, but is prone to error).Īt this point, we are going to get into the actual DNA analysis on DSAP. Click on “My Clones” and then click on “PC1.11” Log into you DSAP homepage like last time. When you get there, click the green button “Next”. When you have clicked that button, it should take you to a page that looks like this.
When we say readable what we mean is: Check what the machine reads. Are there clear, evenly spaced peaks in the sequence? Does the machine have many “N” nucleotides (ones that it can’t figure out what it is)? Is there “noise” beneath the peaks of the sequence? Though the machine may say that the sequence is what it is, check if there are other peaks beneath the sequence that shouldn’t be there. Does the middle of the sequence look like that? Are the wavelengths just plain sloppy? If you look to the beginning and to the very end, you’ll see that the peaks are “sloppy” and uneven. If your sequence has any of those, then it’s most likely unreadable. If you think it’s unreadable (I sort of gave it away), then when you click “next”, then it takes you to the analysis page. There will be two questions asking why you think it’s unreadable. We will go over possible answers to the question next meeting Think about the questions, but don’t answer them yet. In the meantime, go back to “My Clones” and download PC2.11.
If you want, open 4peaks or Finch TV and see what you can make of that sequence. Sorry if the instructions are kind of confusing and for tacking homework on you, but there’s only so much you can get done in half-an-hour (not alot). If you can do this for Monday, that would be great. Each post should take no more than 10-15 minutes to read, but please pay attention and try to understand, because you’ll eventually have to do this yourselves eventually. If you don’t, I can always better clarify things in person. Today, we’ll be “cropping the ends” of our sequenced genome. Why can’t we just take the sequence as it is? What this means is that we’ll be taking the nucleotides off of the sequence that arent part of the Wolffia Australiana gene that codes for a protein. Well, when you do a PCR Reaction, you’re replicating DNA. DNA replication (as the sophomores know, and the freshmen will soon find out) occurs when a primer “sits” down on the DNA sequence and stimulates copying of the gene nucleotide for nucleotide. Long story short, there are excess nucleotides on the sequenced DNA sequence. This includes the promoter sequence, the poly-A tail, and excess before and beyond them. What this means is that we’re going to remove the excess sequence, starting at the end of the promoter sequence, and ending at the last “A” in the Poly-A tail.īefore we move on, however, a note on sequencing: The Poly-A Tail is a string of adenines (one of the 4 types of nucleotides) that signals the end of a protein sequence. To sequence a gene (the highly simplified verison of it), scientists must put the DNA in a solution where the base pairs of nucleotides in the geneome (A-T, and G-C) will pair up with specially manufactured nucleotides that reflect a certain wavelength of light. When the base pairs have matched up with these special nucleotides, it is processed. According to how strong the particular reflection of a wavelength is, the machine will record that nucleotide in the sequence as either A,T,C, or G.
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